Approach

 

To study the molecular architecture and function of neuronal synapses at high temporal and spatial resolution, we use advanced live-cell confocal and super-resolution imaging techniques. In particular, we exploit single-molecule localization and tracking PALM (photoactivated localization microscopy) that allow us to investigate protein organization and motion within individual, living synapses at nanometer resolution.

tracks

We are currently also exploring the possibilities that super-resolution STED (stimulated emission depletion) microscopy and FCS (fluorescence correlation spectroscopy) can offer. These advanced imaging approaches are combined with specific pharmacological and molecular interventions to gain mechanistic insight in the processes that control the molecular organization of synapses.